This server is under virtually continuous development, and has not yet
formally been made public. There is nothing in here that we presently
consider finished, and several of the sections are very much in the
"proof of concept" stage. All textual, image and software material associated
with this server which is original to this site is to be considered
copyrighted, at least by the Regents of the University of Minnesota, and,
in some cases, by other entities.
Ernie Retzel
ernest@lenti.med.umn.edu
Research Projects
Arabidopsis cDNA Sequence Analysis Project
The
Arabidopsis cDNA Sequence Analysis Project is a joint effort
between the University of Minnesota and Michigan State University, and
funded by the National Science Foundation, under grants
BIR 940-2380 and BIR 931-3751.
This project combines both software and database development, and seeks to
provide alternative views into analyzed data.
The Corn cDNA Sequence Analysis Project has been undertaken to transfer some
of the sequence analysis tools developed for Arabidopsis to the cDNA
sequences currently being generated for corn at the University of Arizona.
The Rice cDNA Sequence Analysis Project has been undertaken to transfer some
of the sequence analysis tools developed for Arabidopsis to the cDNA
sequences currently being generated for rice at the Rice Genome Research
Program at STAFF Institute in Tsukuba, Japan.
The Zebrafish Sequence Analysis Project has been undertaken to transfer some
of the sequence analysis tools developed for Arabidopsis to the DNA
sequences currently available for zebrafish.
While the polymerase chain reaction provides an extremely powerful tool
for the analysis of populations of cells, the PCR In Situ extends this
technology to the single cell level. In part due to the newness of the
technique, and in part to respond to demand for detailed information,
pre-prints of articles and in-press book chapters are available here.
The PCR In Situ Cookbook of protocols can be found
here.
The Hidden Layers: A Fluid Molecular Biology Didactic Platform
Teaching a course on state of the art molecular biology is challenging
as the underlying technologies advance faster than print support media can
accomodate. In addition, the essential computational tools are generally
ignored as the difficulty of supporting multiple platforms and capacities
are virtually insurmountable. This project seeks to remove the barriers
by using net-based resources [Mosaic], desktop publishing technologies,
client-server computing, device-independent graphics and imaging and
an array of computational tools operating on remote servers.
The Staden software package provides an X-windows-based environment for
sequence analysis and manipulation, which is particularly good for
handling fluorescence-based DNA sequencing projects. The paper-based manual
has been converted to an online document.